Andres Iniesta was taken off in Barcelona's win against Las Palmas with a muscle problem.
The club have since confirmed that the midfielder suffered a hamstring problem and will be out for approximately 10 days.
Therefore, Iniesta will not join up with the Spain squad but could be fit for Barça's game against Atletico on Oct. 14.
Iniesta was one of the players called up by Julen Lopetegui for this week's games against Albania and Israel, both of which are qualifiers for the World Cup in Russia next summer.
Spain are almost assured of their place in Russia due to their recent win over Italy, but still need to see out these two games.
Iniesta will remain in Barcelona working on his recovery, with hopes that he will be fit for Barça's first ever visit to Atletico's new home.